Broad-Range (Pan) Salmonella and Salmonella Serotype Typhi-Specific Real-Time PCR Assays
نویسندگان
چکیده
منابع مشابه
a rapid and specific pcr–elisa for detecting salmonella typhi
background salmonella continues to be a major food borne pathogen for animals and humans. a sensitive and specific pcr–elisa technique was developed to detect salmonella typhi. materials and methods the assay was based on the incorporation of digoxigenin-labeled dutp and a biotin-labeled primers specific for rfbe gene during pcr amplification. the labeled pcr products were bound to streptoavidi...
متن کاملMultiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium
Salmonellosis outbreaks involving typhoid fever and human gastroenteritis are important diseases in tropical countries where hygienic conditions are often not maintained. A rapid and sensitive method to detect Salmonella spp., Salmonella Typhi and Salmonella Typhimurium is needed to improve control and surveillance of typhoid fever and Salmonella gastroenteritis. Our objective was the concurren...
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For epidemiologic investigations, the primary subdivision of Salmonella Typhi is vi-phage typing; 106 Vi-phage types are defined. For multidrug-resistant strains the most common types have been M1 (Pakistan) and E1 (India, Pakistan, Bangladesh, and the Arabian Gulf); a strain untypable with the Vi phages has been responsible for a major epidemic in Tajikistan. Most often, isolates from the Indi...
متن کاملCiprofloxacin-Resistant Salmonella enterica Serotype Typhi, United States, 1999–2008
We report 9 ciprofloxacin-resistant Salmonella enterica serotype Typhi isolates submitted to the US National Antimicrobial Resistance Monitoring System during 1999-2008. The first 2 had indistinguishable pulsed-field gel electrophoresis patterns and identical gyrA and parC mutations. Eight of the 9 patients had traveled to India within 30 days before illness onset.
متن کاملEarly diagnosis of typhoid fever by nested PCR for flagellin gene of Salmonella enterica serotype Typhi
BACKGROUND & OBJECTIVES Typhoid fever caused by Salmonella Typhi continues to be a major health problem in spite of the use of antibiotics and the development of newer antibacterial drugs. Inability to make an early laboratory diagnosis and resort to empirical therapy, often lead to increased morbidity and mortality in cases of typhoid fever. This study was aimed to optimize a nested PCR for ea...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2005
ISSN: 0002-9173
DOI: 10.1309/dp0h-y5ut-10hq-w9ym